Product name: Triosephosphate Isomerase (TPI)
Product description: Recombinant Human TPI enzyme (active) produced in Nicotiana benthamiana by Agrobacterium-mediated transient expression.
Host species: Nicotiana benthamiana
Molecular weight: 54 kDa dimer (2x 27 kDa subunits)
Tag: N-terminal His-tag
Uses: For research use only. Examples include SDS-PAGE, Western Blot, functional assays.
Relevance: Involved in gluconeogenesis (carbohydrate biosynthesis) and glycolysis (carbohydrate degradation). TPI catalyses the reversible conversion of dihydroxyacetone phosphate to glyceraldehyde-3-phosphate D-glyceraldehyde. TPI deficiency (TPID) is an autosomal recessive disorder which is characterized by several traits including progressive neuromuscular dysfunction, cardiomyopathy, susceptibility to bacterial infection and congenital haemolytic anaemia.1,2.
Specification:
Purity: >99 % as shown by SDS-PAGE.
Figure 1: reducing 4-20% SDS-PAGE analysis of 5 µg TPI.
Figure 2: Latter stage of TPI activity assay showing linear change in absorption (450 nm) over 15 minutes up until reaction saturation.
Biological activity: Active. >1000 mU/mg. Measuring the conversion of dihydroxyacetone phosphate (DHAP) to glyceradehyde-3-phosphate (GAP).
Endotoxin: Not tested.
Storage
Formulation: Lyophilized from 20 mM Tris-HCl, pH 7.5, 175 mM Trehalose Dihydrate and 0.9 mM DTT.
Shipped: Recombinant proteins are sterile-filtered and provided as lyophilized powder which are shipped at ambient temperature.
Stability & Storage: See COA for detailed storage instructions. Lyophilized materials are stable for up to twelve months from date of receipt at -70℃.
It is recommended that reconstituted protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instructions is included with the product.
References:
- https://www.uniprot.org/uniprot/P60174
- Orosz F, Olàh J, Ovádi J. Triosephosphate isomerase deficiency: new insights into an enigmatic disease. 1792(12):1168-74 (2009)
