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Product name: Ara h 2 – Arachis hypogaea (Peanut) allergen 2 / Conglutin-7
Product description: Recombinant Arachis hypogaea allergen 2 produced in Nicotiana benthamiana by Agrobacterium-mediated transient expression.
Host species: Nicotiana benthamiana
Molecular weight: 18.8 kDa
Tag: C-terminal His-tag
Uses: For in vitro research use only. Not for human in vivo or therapeutic use.
Relevance: Ara h 2 was the second major peanut allergen to be identified2. Ara h 2 is a monomeric protein that contains 8 cysteine residues that are capable of forming 4 disulfide bonds. Certain forms of the protein have significant homology to trypsin inhibitors and exhibit varying degrees of trypsin inhibition3. Ara h 2 has been demonstrated to effectively cross-link IgE and contributes towards effector activity of crude peanut extract in immunoglobulin E-mediated immune cell activation4.
Purity: >90 % as shown by SDS-PAGE
Figure 1: reducing 4-20% SDS-PAGE analysis of 5 µg Ara h 2.
Biological activity: Not tested.
Endotoxin: Not tested.
Formulation: Lyophilized from 20 mM Tris-HCl, pH 7.5, 175 mM Trehalose Dihydrate.
Shipping: Recombinant proteins are sterile-filtered and provided as lyophilized powder which are shipped at ambient temperature.
Stability & Storage: See COA for detailed storage instructions. Lyophilized materials are stable for up to twelve months from date of receipt at -70℃.
It is recommended that reconstituted protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instructions is included with the product.
- Burks AW, Williams LW, Connaughton C, Cockrell G, O’Brien TJ, Helm RM. Identification and characterization of a second major peanut allergen, Ara h II, with use of the sera of patients with atopic dermatitis and positive peanut challenge. J Allergy Clin Immunol. 90:962-9 (1992)
- Maleki SJ, Viquez O, Jacks T, Dodo H, Champagne ET, Chung SY, Landry SJ. The major peanut allergen, Ara h 2, functions as a trypsin inhibitor, and roasting enhances this function. J Allergy Clin Immunol. 112:190-195 (2003)
- McDermott RA, Porterfield HS, El Mezayen R, Burks AW, Pons L, Schlichting DG, Solomon B, Redzic, JS, Harbeck RJ, Duncan MW, Hansen KC, Dreskin SC. Contribution of Ara h 2 to peanut-specific, immunoglobulin E-mediated, cell activation. Clin Exp Allergy. 37:752-763 (2007)