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Product name: Ara h 2 – Arachis hypogaea (Peanut) allergen 2 / Conglutin-7

Product description: Recombinant Arachis hypogaea allergen 2 produced in Nicotiana benthamiana by Agrobacterium-mediated transient expression.

Host species: Nicotiana benthamiana

Molecular weight: 18.8 kDa

Tag: C-terminal His-tag

Uses: For in vitro research use only. Not for human in vivo or therapeutic use.

Relevance: Ara h 2 was the second major peanut allergen to be identified2. Ara h 2 is a monomeric protein that contains 8 cysteine residues that are capable of forming 4 disulfide bonds. Certain forms of the protein have significant homology to trypsin inhibitors and exhibit varying degrees of trypsin inhibition3. Ara h 2 has been demonstrated to effectively cross-link IgE and contributes towards effector activity of crude peanut extract in immunoglobulin E-mediated immune cell activation4.

Specification

Purity: >90 % as shown by SDS-PAGE

 

 

 

 

 

 

 

 

 

Figure 1: reducing 4-20% SDS-PAGE analysis of 5 µg Ara h 2.

Biological activity: Not tested.

Endotoxin: Not tested.

Storage

Formulation: Lyophilized from 20 mM Tris-HCl, pH 7.5, 175 mM Trehalose Dihydrate.

Shipping: Recombinant proteins are sterile-filtered and provided as lyophilized powder which are shipped at ambient temperature.

Stability & Storage: See COA for detailed storage instructions. Lyophilized materials are stable for up to twelve months from date of receipt at -70℃.

It is recommended that reconstituted protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution: A hardcopy of COA with reconstitution instructions is included with the product.

 

References

  1. https://www.uniprot.org/uniprot/Q6PSU2
  2. Burks AW, Williams LW, Connaughton C, Cockrell G, O’Brien TJ, Helm RM. Identification and characterization of a second major peanut allergen, Ara h II, with use of the sera of patients with atopic dermatitis and positive peanut challenge. J Allergy Clin Immunol. 90:962-9 (1992)
  3. Maleki SJ, Viquez O, Jacks T, Dodo H, Champagne ET, Chung SY, Landry SJ. The major peanut allergen, Ara h 2, functions as a trypsin inhibitor, and roasting enhances this function. J Allergy Clin Immunol. 112:190-195 (2003)
  4. McDermott RA, Porterfield HS, El Mezayen R, Burks AW, Pons L, Schlichting DG, Solomon B, Redzic, JS, Harbeck RJ, Duncan MW, Hansen KC, Dreskin SC. Contribution of Ara h 2 to peanut-specific, immunoglobulin E-mediated, cell activation. Clin Exp Allergy. 37:752-763 (2007)